Table 1.
Primer sequences and product sizes for semiquantitative RT-PCR.
| Gene | Primer Sequences (5’–3’) | Annealing Temperature (°C) | Product Size (Base Pairs) | Cycles | MgCl2 |
|---|---|---|---|---|---|
| housekeeping gene for internal control | |||||
| EEF1A1 | Sense: AGGTGATTATCCTGAACCATCC Antisense: AAAGGTGGATAGTCTGAGAAGC |
54.0 | 234 | 21 | 1 x |
| osteogenic marker genes | |||||
| COL1A1 | Sense: GGACACAATGGATTGCAAGG Antisense: TAACCACTGCTCCACTCTGG |
55.0 | 461 | 22 | 2x |
| COL10A1 | Sense: CCCTTTTTGCTGCTAGTATCC Antisense: CTGTTGTCCAGGTTTTCCTGGCAC |
54.0 | 468 | 40 | 1x |
| ALP | Sense: TGGAGCTTCAGAAGCTCAACACCA Antisense: ATCTCGTTGTCTGAGTACCAGTCC |
51.0 | 454 | 33 | 1x |
| OC | Sense: ATGAGAGCCCTCACACTCCTC Antisense: GCCGTAGAAGCGCCGATAGGC |
62.0 | 293 | 35 | 2x |
| chondrogenic marker genes | |||||
| ACAN | Sense: GCCTTGAGCAGTTCACCTTC Antisense: CTCTTCTACGGGGACAGCAG |
54.0 | 400 | 35 | 1x |
| COL2A1 | Sense: TTTCCCAGGTCAAGATGGTC Antisense: CTTCAGCACCTGTCCACCA |
51.0 | 155 | 31 | 1x |
| SOX9 | Sense: ATCTGAAGAAGGAGAGCGAG Antisense: TCAGAAGTCTCCAGAGCTTG |
60.0 | 263 | 31 | 1x |
| COMP | Sense: CAGGACGACTTTGATGCAGA Antisense: AAGCTGGAGCTGTCTGGTA |
54.0 | 312 | 32 | 1x |