Figure 1.

(A) Representative immunoblot with serum of a hunter. The sample was probed for the presence of IgG antibodies against lysates of the E. coli expression strain Bl21(DE3)pLysS and different Borrelia species as well as purified recombinant proteins Vsp1 and GlpQ. E. coli strain Bl21(DE3)pLysS; B.a., B. afzelii strain 1960/11; B.t., B. turicatae; B.m. HT31, B. miyamotoi strain HT31; Vsp1 of different purities (E1 and E4); GlpQ-N, N-terminally 6xHis-tagged version of GlpQ produced in the Vienna laboratory; GlpQ_DIA, recombinant GlpQ produced by DIARECT. (B) Immunoblot of the same serum sample to test whether the position of the His-tag (GlpQ-N, N-terminal 6x His-tag; GlpQ-C, C-terminal 6x His-tag) has influence on reactivity.