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. 2020 Nov 20;14(12):16414–16445. doi: 10.1021/acsnano.0c07638

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© 2020 American Chemical Society

This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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(A) Photothermal image of four constructs (BHQ1-10T-BHQ1) in nitrogen-bubbled glycerol, each consisting of two BHQ1 molecules and one single strand of DNA with 10 thymine (T) bases. (B) Photothermal time traces of single BHQ1-10T-BHQ1 in an aerated environment showing single-step photobleaching. (C) Histogram of the change of absorption cross sections calculated from 30 one-step bleaching events. The average cross section is 0.041 nm². The solid red line represents the distribution of absorption cross sections of single chromophores considering an isotropic distribution of transition dipole moments. Top: Chemical structure of a BHQ1 molecule. (D) Histogram of survival times before single step bleaching in aerated environment. The average survival time is 49 s. Reprinted with permission from ref (22). Copyright 2010 The American Association for the Advancement of Science.