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. 2020 Dec 11;12(51):56723–56730. doi: 10.1021/acsami.0c16208

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© 2020 American Chemical Society

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Stem cell secretome is influenced by matrix properties. (a) Quantification of the stem cell secretome by the multiplex assay. The heat map (left) shows fold increase in cytokine expression of S2, S3, and S4 in comparison to S1. Cytokines with marked higher expressions in RGD-appended matrices are labeled in pink; IL-10, which is higher expressed in inert matrices, is labeled in dark green. The bar chart (right) shows the absolute concentrations of cytokines in S1 and the medium control. See Figure S2 for the absolute expression levels. Cell density: 500,000 cells/mL; conditioned medium was refreshed every 2 days, and the medium at day 6 was collected for the measurement. (b) ELISA reveals the average concentration of IL-10 in CM (left) and normalized per cell (right) for all samples. Cell density: 200,000 cells/mL; conditioned medium was refreshed on day 1, 3, and 7; the medium at day 7 was collected for the measurement.