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. 2020 Nov 27;12(12):3546. doi: 10.3390/cancers12123546

Figure 2.

Figure 2

MYC-induced miR-378a-3p is essential for BL cell growth. (A) Levels of MYC and miR-378a-3p in tetracycline treated (“−”, MYC-off) and non-treated (“+”, MYC-on) P493-6 B-cells. MYC levels were normalized to RNA Polymerase II Subunit A (POLR2A). MiRNA levels were normalized to SNORD44. (B) Green fluorescent protein (GFP) growth competition assay upon miR-378a-3p inhibition in four Burkitt lymphoma (BL) cell lines. The miR-378a inhibitor (mZip-378a-3p) and the scrambled control (mZip-SCR) were stably transduced in BL cells using a lentiviral vector, which co-expresses GFP. The GFP percentage was measured for 18 days, and the GFP percentage at the first day of measurement (day 4) was set to 100%. All assays were performed in triplicate. Significant differences were calculated using a mixed model analysis. *** p < 0.001 and **** p < 0.0001.