ΔR/R0
|
change in fluorescence ratio, normalized to control ratio |
ACSF |
artificial cerebrospinal fluid |
DIV |
days in vitro |
DMSO |
dimethyl sulfoxide |
DTT |
1,4-dithio-DL-threitol |
DPI |
diphenyleneiodonium chloride |
E0′roGFP
|
standard redox potential |
EroGFP
|
roGFP reduction potential |
F 380max/F 380min
|
fluorescence ratio at 380 nm excitation, Ca2+-free/Ca2+-saturated |
F 395/F 470 |
fluorescence ratio at 395 nm excitation and 470 nm excitation |
F 470ox
|
fluorescence intensity at 470 nm excitation, fully oxidized |
F 470ox/F 470red
|
fluorescence ratio at 470 nm, fully oxidized/fully reduced |
F 470red
|
fluorescence intensity at 470 nm excitation, fully reduced |
FCCP |
carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone |
FCS |
fetal calf serum |
HBSS |
Hanks’-balanced salt solution |
HEPES |
4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid |
MAP2 |
microtubule associated protein 2 |
MeCP2 |
methyl-CpG binding protein 2, protein |
MECP2
|
methyl-CpG binding protein 2, encoding gene (human) |
Mecp2
|
methyl-CpG binding protein 2, encoding gene (mouse) |
Mecp2−/y
|
MeCP2-deficient male mouse (hemizygous) |
Mecp2+/−
|
MeCP2-deficient female mouse (heterozygous) |
MEM |
minimal essential medium |
NOX |
NADPH oxidase |
OxDroGFP
|
relative degree of roGFP oxidation |
PBS |
phosphate buffered saline |
R |
fluorescence ratio |
roGFP |
reduction oxidation-sensitive green fluorescent protein |
roGFPc |
roGFP expressed in cytosol |
roGFPm |
roGFP expressed in mitochondrial matrix |
ROS |
reactive oxygen species |
Rox
|
ratio corresponding to full oxidation |
Rred
|
ratio corresponding to full reduction |
RTT |
Rett syndrome |
SOD1 |
superoxide dismutase 1, Cu/Zn superoxide dismutase |
WT |
wildtype |
XO |
xanthine oxidase |
XDH |
xanthine dehydrogenase |