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. 2020 Nov 24;9(12):2539. doi: 10.3390/cells9122539
ΔR/R0 change in fluorescence ratio, normalized to control ratio
ACSF artificial cerebrospinal fluid
DIV days in vitro
DMSO dimethyl sulfoxide
DTT 1,4-dithio-DL-threitol
DPI diphenyleneiodonium chloride
E0′roGFP standard redox potential
EroGFP roGFP reduction potential
F 380max/F 380min fluorescence ratio at 380 nm excitation, Ca2+-free/Ca2+-saturated
F 395/F 470 fluorescence ratio at 395 nm excitation and 470 nm excitation
F 470ox fluorescence intensity at 470 nm excitation, fully oxidized
F 470ox/F 470red fluorescence ratio at 470 nm, fully oxidized/fully reduced
F 470red fluorescence intensity at 470 nm excitation, fully reduced
FCCP carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
FCS fetal calf serum
HBSS Hanks’-balanced salt solution
HEPES 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid
MAP2 microtubule associated protein 2
MeCP2 methyl-CpG binding protein 2, protein
MECP2 methyl-CpG binding protein 2, encoding gene (human)
Mecp2 methyl-CpG binding protein 2, encoding gene (mouse)
Mecp2−/y MeCP2-deficient male mouse (hemizygous)
Mecp2+/− MeCP2-deficient female mouse (heterozygous)
MEM minimal essential medium
NOX NADPH oxidase
OxDroGFP relative degree of roGFP oxidation
PBS phosphate buffered saline
R fluorescence ratio
roGFP reduction oxidation-sensitive green fluorescent protein
roGFPc roGFP expressed in cytosol
roGFPm roGFP expressed in mitochondrial matrix
ROS reactive oxygen species
Rox ratio corresponding to full oxidation
Rred ratio corresponding to full reduction
RTT Rett syndrome
SOD1 superoxide dismutase 1, Cu/Zn superoxide dismutase
WT wildtype
XO xanthine oxidase
XDH xanthine dehydrogenase