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. 2020 Nov 26;11(12):832. doi: 10.3390/insects11120832

Figure 5.

Figure 5

Electron micrographs showing the process of encapsulation of S. abbasi in the 4th instar larvae of Ae. albopictus. (a) At 10 min after inoculation, melanotic capsule deposited on the surface. C: the cuticle of S. abbasi; M: melanotic capsule; Sa: S. abbasi. (b) At 10 min after inoculation, the electron-condense homogeneous material attached to the cuticle of S. abbasi and symbiotic bacteria-like structures around S. abbasi in the hemocoel of 4th instar larvae. Arrow head (▲): bacteria-like structures; Sa: S. abbasi. C: the cuticle of S. abbasi. (c) At 30 min after inoculation, the inner electron-dense material thickened. Arrows (↑): the electron-condense homogeneous materials; M: melanotic capsule; Sa: S. abbasi. (d) At 1 h after inoculation, cell debris appeared on the out layer of humoral capsule. Black arrows (↑): mitochondria; White arrows (↑): cell debris; M: melanotic capsule. (e) At 1 h after inoculation. M: melanotic capsule; P: plasmatocyte; Arrows (↑): mitochondria. (f) At 2 h after inoculation, plasmatocyte on the melanotic capsule. C: the cuticle of S. abbasi; M: melanotic capsule; P: plasmatocyte; Sa: S. abbasi. (g) At 24 h after inoculation, intact hemocytes appeared on the humoral capsule. Arrow head: a hemocyte. (h) At 48 h after inoculation, the basement membrane-like structures between cellular capsule and hemocoel. Arrow head: the basement membrane-like structures.