Table 2.
Deiminated proteins in hemolymph of soft shell clam (Mya arenaria), as identified by F95-enrichment in conjunction with LC–MS/MS analysis. Deiminated proteins were isolated from hemolymph (a pool of hemolymph from four individual animals) by immunoprecipitation using the pan-deimination F95 antibody. The resulting F95-enriched eluate was then analyzed by LC–MS/MS and peak list files submitted to Mascot, using both a species-specific as well as a common Mollusca database. Peptide sequence hits are listed, showing species-specific hit, number of sequences for protein hits, and total score. Species hit names are indicated, soft shell clam specific hits are on the top of the list and highlighted. * Proteins only identified in soft shell clam. (See Table S2 for full details on all protein hits).
| Protein ID Protein Name |
Species Name Common Name |
Matches (Sequences) |
Total Score (p < 0.05) ‡ |
|---|---|---|---|
| V9VED0_MYAAR Actin (Fragment) |
Mya arenaria Soft shell clam |
7 (5) |
255 |
| Q6YNF3_MYAAR Histone H3 (Fragment) |
Mya arenaria Soft shell clam |
4 (2) |
90 |
| J9Z3Z3_MYAAR Elongation factor 1 alpha |
Mya arenaria Soft shell clam |
2 (1) |
38 |
| A0A0L8HIZ8_OCTBM Uncharacterized protein (actin) |
Octopus bimaculoides California two-spot octopus |
14 (7) |
448 |
| A0A6J8C382_MYTCO H2A |
Mytilus coruscus Korean mussel |
4 (2) |
188 |
| A0A6J8AIH4_MYTCO H3 |
Mytilus coruscus Korean mussel |
6 (4) |
164 |
| A0A0B7B588_9EUPU Tubulin alpha chain |
Arion vulgaris Spanish slug |
2 (2) |
150 |
| A0A077D3S6_MYTGA Glyceraldehyde-3-phosphate dehydrogenase |
Mytilus galloprovincialis Mediterranean mussel |
3 (1) |
140 |
| V4A0D9_LOTGI Histone H4 |
Lottia gigantean Owl limpet |
5 (2) |
108 |
| A0A6J7ZUB9_MYTCO PGK |
Mytilus coruscus Korean mussel |
2 (2) |
94 |
| A0A499QNG2_RUDPH Receptor for activated C kinase |
Ruditapes philippinarum Manila clam |
3 (1) |
75 |
| H6BD30_OSTED GAPDH glyceraldehyde 3 phosphate dehydrogenase |
Ostrea edulis European flat oyster |
8 (1) |
71 |
| A0A077GY54_MYTTR EP protein |
Mytilus trossulus Bay mussel |
1 (1) |
68 |
| A0A194ALQ1_PINFU Elongation factor 1-alpha |
Pinctada fucata Akoya pearl oyster |
3 (2) |
65 |
| A0A077GYT3_MYTTR Cold-shock domain protein |
Mytilus trossulus Bay mussel |
1 (1) |
59 |
|
A0A0R6BQX1_CRAHO Superoxide dismutase |
Crassostrea hongkongensis | 1 (1) |
56 |
| K1QK39_CRAGI *Dynein heavy chain 2, axonemal |
Crassostrea gigas Pacific oyster |
7 (2) |
56 |
| V4AES5_LOTGI *Uncharacterized protein |
Lottia gigantean Owl limpet |
1 (1) |
54 |
| A0A2T7NYL0_POMCA Uncharacterized protein |
Pomacea canaliculata Channeled applesnail |
1 (1) |
50 |
| A0A6J8AYS7_MYTCO Uncharacterized protein |
Mytilus coruscus Korean mussel |
5 (1) |
48 |
| A0A210R3U3_MIZYE *80 kDa MCM3-associated protein |
Mizuhopecten yessoensis Yesso/Ezo scallop |
27 (2) |
46 |
| A0A6J8D0T9_MYTCO PARP7S |
Mytilus coruscus Korean mussel |
2 (1) |
44 |
| A0A6J8BH71_MYTCO *SCRN |
Mytilus coruscus Korean mussel |
1 (1) |
42 |
| A0A3S1AG64_ELYCH *Uncharacterized protein |
Elysia chlorotica Eastern emerald elysia |
20 (1) |
41 |
‡ Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 41 indicate identity or extensive homology (p < 0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.