Table 4.
Deiminated proteins in hemolymph of Atlantic jacknife clam (Ensis leei), as identified by F95-enrichment followed by LC–MS/MS analysis. Deiminated proteins were isolated from hemolymph (a pool of hemolymph from four individual animals) by immunoprecipitation using the pan-deimination F95 antibody. The resulting F95-enriched eluate was then analyzed by LC–MS/MS and peak list files submitted to Mascot, using both a species-specific as well as a common Mollusca database. Peptide sequence hits are listed, showing species-specific hit, number of sequences for protein hits, and total score. * Proteins only identified in Atlantic jacknife clam. (See Table S4 for full details on all protein hits).
| Protein ID Protein Name |
Species Name Common Name |
Matches (Sequences) |
Total Score (p < 0.05) ‡ |
|---|---|---|---|
| A0A6J8CKZ0_MYTCO Histone H4 |
Mytilus coruscus Korean mussel |
9 (7) |
314 |
| A0A6J8C382_MYTCO Histone H2A |
Mytilus coruscus Korean mussel |
9 (7) |
312 |
| A0A1B1H1S7_9CAEN Histone H3 (Fragment) |
Fonscochlea zeidleri Freshwater snail |
6 (5) |
200 |
| V4A0D9_LOTGI Histone H4 |
Lottia gigantea Owl limpet |
5 (4) |
186 |
| A0A076FHY5_PATCE Beta-actin (Fragment) |
Patella caerulea Mediterranean limpet |
5 (2) |
170 |
| A0A1V4JT39_PATFA Glyceraldehyde-3-phosphate dehydrogenase |
Mytilus galloprovincialis Mediterranean mussel |
2 (1) |
111 |
| A0A0K0PUN7_MYTGA *Nacre c1q domain-containing protein 1 |
Mytilus galloprovincialis Mediterranean mussel |
2 (2) |
101 |
| Q0KJW4_CRAVI Dominin |
Crassostrea virginica Eastern Oyster |
4 (2) |
85 |
| A0A0R6BQX1_CRAHO Superoxide dismutase |
Crassostrea hongkongensis Hong Kong Oyster |
3 (1) |
55 |
| K1PY89_CRAGI Extracellular superoxide dismutase |
Crassostrea gigas Pacific Oyster |
3 (1) |
55 |
| H9ZXX0_CRAVI Major plasma protein 2 |
Crassostrea virginica Eastern Oyster |
1 (1) |
84 |
| A0A6J7ZZ73_MYTCO *ACTB_G1 |
Mytilus coruscus Korean mussel |
2 (2) |
75 |
| A0A1B2CWA8_FUSFL Elongation factor 1-alpha |
Fusconaia flava Wabash pigtoe |
2 (2) |
72 |
| H6BD30_OSTED GAPDH glyceraldehyde 3 phosphate dehydrogenase |
Ostrea edulis European flat oyster |
3 (1) |
69 |
| A0A6J8D0T9_MYTCO PARP7S |
Mytilus coruscus Korean mussel |
3 (1) |
56 |
| A0A2T7NYL0_POMCA Uncharacterized protein |
Pomacea canaliculata Channeled applesnail |
2 (1) |
55 |
| A0A0A7HG21_POMCA *Heat shock protein 90 |
Pomacea canaliculata Channeled applesnail |
1 (1) |
53 |
| A0A0B7B588_9EUPU Tubulin alpha chain |
Arion vulgaris Spanish slug |
1 (1) |
49 |
| A0A6J8AYS7_MYTCO Uncharacterized protein |
Mytilus coruscus Korean mussel |
3 (1) |
48 |
| A0A076KW18_MYTGA Ubiquitin C |
Mytilus galloprovincialis Mediterranean mussel |
1 (1) |
43 |
| A0A210R431_MIZYE Sequestosome-1 |
Mizuhopecten yessoensis Yesso scallop |
1 (1) |
43 |
| A0A0L8HUL5_OCTBM *PDZ domain-containing protein |
Octopus bimaculoides California two-spot octopus |
1 (1) |
41 |
| K1QE77_CRAGI Solute carrier family 25 member 40 |
Crassostrea gigas Pacific oyster |
1 (1) |
41 |
| A0A6J8A7V5_MYTCO *Uncharacterized protein |
Mytilus coruscus Korean mussel |
3 (1) |
40 |
‡ Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 41 indicate identity or extensive homology (p < 0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.