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. 2020 Nov 30;9(12):1201. doi: 10.3390/antiox9121201

Figure 4.

Figure 4

BPA amplifies immune response and cytokine transcription and induces inflammasome activation in the liver of HFD mice. Protein expression of (A) TLR4 and mRNA levels of (B) Myd88, and (C) Nfkb1 were determined in the liver from all mice. mRNA transcript of (D) Tnfa, Il6, and Ifng was measured. The activation of hepatic inflammasome complexes by BPA was shown by the marked increase of (E) NLRP3 protein expression, and the upregulation of (F) Nlrp3, Casp1, Pycard, and Il1b transcripts, evaluated by Western blot and real-time PCR, respectively. Cropped Western blots for TLR4 and NLRP3 were shown. The increase of chemokine-guided monocyte recruitment induced by BPA was demonstrated by the evaluation of mRNA transcription of (G) Ccl2 and Itgax. Data are presented as means ± SEM of all animals (n = 6 each group) (* p < 0.05, and *** p < 0.001 vs. STD; # p < 0.05, ## p < 0.01, and #### p < 0.0001 vs. HFD).