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. 2020 Nov 12;6(12):2301–2310. doi: 10.1021/acscentsci.0c01146

Figure 4.

Figure 4

Colocalization of endogenous nucleic acids with supercationic GFP condensates. (a) The colocalization of DNA with the GFP(+36) condensates was evaluated by staining cells with DAPI (a DNA-specific dye). Microscopy images depict cells expressing GFP(+36) or sfGFP and stained with DAPI. Intensity line-cuts demonstrate exclusion of DNA from the GFP(+36) condensates. (b) The colocalization of RNA with the GFP(+36) condensates was evaluated by staining cells with SYTO 17, which binds both DNA and RNA. Microscopy image of cells expressing GFP(+36) or sfGFP and stained with SYTO 17 are shown along with intensity line-cuts demonstrating colocalization of RNA and GFPs. Scale bars, 2 μm.