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. 2020 Nov 12;6(12):2301–2310. doi: 10.1021/acscentsci.0c01146

Figure 5.

Figure 5

Colocalization of endogenous proteins with supercationic GFP condensates. (a) Proteins from lysed E. coli cells enriched following high salt fractionation were quantified by LC–MS/MS. The fold enrichment under high salt treatment is plotted against the predicted protein charge. (b) Selected proteins from (a) were coexpressed with GFP(+36) as mScarlet-I fusions. Fluorescence microscopy and intensity line-cuts demonstrate colocalization of GFP(+36) with hldD and rraB mScarlet-I fusions. Additional coexpression data is found in Supplementary Figure 23. Scale bars, 2 μm.