Figure 4.

meCLICK-Seq reveals widespread m⁶A mark in introns and intergenic regions. (a) Intronic peaks in the first intron of FLI1. (b) Intronic peaks in the first intron of CADM1 in three isogenic cell models. Intronic peaks are abolished specifically in METTL16-KD cells. (c) Overlap between METTL3- and METTL16-dependent intronic peaks. (d) Distribution of METTL3-dependent peaks in intronic and intergenic regions. (e) Distribution of METTL16-dependent peaks in intronic and intergenic regions. (f) RT-qPCR-based validation of a panel intronic peaks, n = 3. (g) Validation of dependence of intronic peaks on RNA methylases METTL3 and METTL16, n = 3. (h) Results of m⁶A-RIP in cells with methylated introns removed by dual gRNA system, n = 3. (i) Results of m⁶A-RIP in cells with depleted METTL3, n = 3. (j) Results of m⁶A-RIP in cells with depleted METTL16. RASA3 peak 2 is not affected by the knock-down illustrating that the observed effect is enzyme-specific, n = 3. ns = not significant (p ≥ 0.05). Error bars represent SD.