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. 2020 Nov 26;12(12):3631. doi: 10.3390/nu12123631

Figure 2.

Figure 2

Gnetin C induces cytotoxicity in DU145 and PC3M prostate cancer cells more potently than Res and Pter. (A,B) A dose-dependent (5–100 µM) cell viability assay was performed after 72 h of treatment with compounds. Viable cells were plotted as percent of vehicle treated cells (Ctrl), which was set as 100%. (C,D) Effects of Gnetin C, Res and Pter on prostate cancer cell proliferation. Cells were plated in phenol-red-free media supplemented with 5% charcoal-stripped serum and cultured in the presence of 5 μM of compounds for 10 days. (E,F) Analysis after treatment with compounds was performed using Flow Cytometry and sub-G1 population was calculated. Data represent the mean ± SEM of three independent experiments. Data represent the mean ± SEM of three independent experiments, in which each data point was performed in triplicates. * p < 0.05; *** p < 0.001; **** p < 0.0001 (significance vs. Gnetin 25); ### p < 0.001; #### p < 0.0001 (significance vs. Gnetin 50) (two-way ANOVA).