Figure 3.

Gnetin C reduces clonogenic survival and motility in DU145 and PC3M prostate cancer cells more potently than Res and Pter. (A,B) Representative images (left) and quantification (right) of the number of colonies showing colony formation ability of prostate cancer cells after treatment with 5 μM of compounds for 14 days. Data represent the mean ± SEM of three independent experiments with duplicate wells. ** p < 0.01; *** p < 0.001; **** p < 0.0001 (one-way ANOVA). (C,D) Representative images (left) and quantification (right) of the migration ability of cells after treatment with 1 µM of compounds for 48 h. Values of vehicle-treated Ctrl cells at 0 h were assigned as 100%, and the percentage of wound area of compound-treated cells was calculated on that base. 10X images (scale bar, 100 µm). Data represent the mean ± SEM of six separate wound areas from three independent experiments. ** p < 0.01; *** p < 0.001; **** p < 0.0001 (one-way ANOVA).