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Microfluidic design pattern and laser cutting of device’s PMMA layer (t: top layer, m: middle layer, b: bottom layer). (a) Two-layers device. (b) Schematic of the cross-section view of the Two-layers device (channel width: 700 µm). (c) Three-layers device. (d) Schematic of the cross-section view of the Three-layers device (channel width: 700 µm).
