Figure 1.

Extraction protocols evaluated for metabolomic and lipidomic profiling of colorectal cancer (CRC) organoids using LC-QTOF-MS after dual LC separation by HILIC and RPLC. The number of seeded cells/well was determined using an hemocytometer. Cell numbers after incubation were approximated based on the doubling time (3.4 days) as determined in concomitant experiments. Cell numbers for LC-MS analysis were calculated based on the solvent volumes used for sample preparation (optimized protocol C, see Supplementary Figure S7). Resulting extracts were dried and reconstituted in appropriate solvent prior to LC-QTOF-MS analysis.