Table 1.
Number of metabolites with significantly and relevantly higher abundance in organoid samples compared to respective controls (ECM only) following different sample preparation protocols and LC QTOF-MS methods in the indicated electrospray ionization (ESI) mode.
| Protocol | Analytical Mode | No. of Significant and Relevant Metabolites (Organoids vs. ECM Controls) | Median CV of Significant and Relevant Metabolites (%) |
|---|---|---|---|
| A | RPLC ESI (−) | 17 | 21.7 |
| RPLC ESI (+) | 12 | 14.7 | |
| B | RPLC ESI (−) | 13 | 7.0 |
| RPLC ESI (+) | 13 | 8.9 | |
| A/B 1 | HILIC ESI (−) | 15 | 25.7 |
| HILIC ESI (+) | 19 | 33.5 | |
| C | RPLC ESI (−) | 44 | 13.6 |
| RPLC ESI (+) | 54 | 10.4 | |
| HILIC ESI (−) | 17 | 26.8 | |
| HILIC ESI (+) | 25 | 16.2 |
1 Data of protocols A and B was combined for statistical evaluation, as sample preparation for both protocols is identical in the HILIC mode, see Figure 1.