Table 2.
The selected genes and their primer sequences.
| Sr No | Target Genes | Primers | Sequences |
|---|---|---|---|
| Housekeeping Gene | |||
| 1 | Glyceraldehyde-3-phosphate dehydrogenase | Forward Reverse |
AGTTCAACGGCACAGTCAAG TACTCAGCACCAGCATCACC |
| Transcription Factors | |||
| 1 | Sterol Regulatory Element-Binding Proteins-1c | Forward Reverse |
AAACCTGAAGTGGTAGAAAC TTATCCTCAAAGGCTGGG |
| 2 | Peroxisome Proliferator-Activated Receptor Gamma | Forward Reverse |
AAGACAACAGACAAATCACC CAGGGATATTTTTGGCATACTC |
| 3 | Nuclear Factor-κβ | Forward Reverse |
AAAAACGAGCCTAGAGATTG ACATCCTCTTCCTTGTCTTC |
| Fatty Acid Metabolism Genes | |||
| 1 | Fatty Acid Synthase | Forward Reverse |
AAAAGGAAAGTAGAGTGTGC GACACATTCTGTTCACTACAG |
| 2 | Acetyl-CoA Carboxylase Alpha | Forward Reverse |
AGCAGTATTTGAACACATGG CAGTTCCAAGAAGTAGAAGC |
| 3 | Carnitine Palmitoyltransferase 1 | Forward Reverse |
CACTGATGAAGGAAGAAGAC CCAGTCACTCACGTAATTTG |
| 4 | Fatty Acid Binding Protein | Forward Reverse |
TGGAGGGTGACAATAAAATG TCATGGTATTGGTGATTGTG |
| Inflammatory Marker | |||
| 1 | Tumor Necrosis Factors-α | Forward Reverse |
CTCACACTCAGATCATCTTC GAGAACCTGGGAGTAGATAAG |