Table 7.
The qRT-PCR efficiency for absolute mRNA quantification.
| No. | Target Genes | Symbol | Efficiency |
|---|---|---|---|
| Housekeeping Gene | |||
| 1 | Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 97.27 |
| Transcription Factors | |||
| 1 | Sterol Regulatory Element-Binding Proteins-1c | SREBP-1c | 91.14 |
| 2 | Peroxisome Proliferator-Activated Receptor Gamma | PPAR-γ | 91.81 |
| 3 | Nuclear Factor-κβ | NF-κβ | 101.90 |
| Fatty Acid Metabolism Genes | |||
| 1 | Fatty Acid Synthase | FASN | 91.67 |
| 2 | Acetyl-CoA Carboxylase Alpha | ACACA | 84.87 |
| 3 | Carnitine Palmitoyltransferase 1 | CPT 1 | 101.65 |
| 4 | Fatty Acid Binding Protein | FABP | 92.96 |
| Inflammatory Marker | |||
| 1 | Tumor Necrosis Factors-α | TNF-α | 107.09 |
Efficiency is calculated from the slope of the curve as E = 10(−1/slope)−1.