Figure 4.

Visible graphic differences in absorption of glycated nail proteins in the region of interest (black arrow) by ATR–FTIR spectroscopy between groups in diabetes mellitus (DM) and digital retinopathy (DR) group (upper build) and control group (lower build). Graphic on upper build represents differences of absorption before and after exposure to the enzyme in DM group (black curve: absorption in vitro glycation 90.7%, red curve: absorption in vitro deglycation 73.6%) in comparison with control group (black curve: absorption in vitro glycation 82.4%, red curve: absorption in vitro deglycation 78.6%).