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Relative expression of selected targets validating RNAseq data. For A549 cell line, XIST, RHOH, PRKCQ, CDKN1A and NrCam were validated using qRT-PCR. Significant downregulation corroborated the RNA-seq data. Similarly, XIST, RHOH, PROX1AS and SFT1AP were also assessed. Downregulation of XIST and SFT1AP was noted and upregulation of RHOH and PROX1AS following siRNA transfection. These data also corroborate the trends seen in RNA-seq for this cell line.
