Table 1.
Primers for targets selected.
| Primer | Sequence 5′–3′ |
|---|---|
| 18S RNA-Forward | ATGGCCGTTCTGAGTTGGTG |
| 18S RNA-Reverse | CGCTGAGCCAGTCAGTGTAG |
| PRKCQ-Forward | CTTGTGGCAGCTTTGGATGT |
| PRKCQ-Reverse | CGTTTCTGACGCACATGTTT |
| NrCAM-Forward | TTGTGCAAAGAGGGAGCATG |
| NrCAM-Reverse | GGGCAGTTCCCTGTTGTCCT |
| CDKN1A-Forward | GCAGACCAGCATGACAGATTT |
| CDKN1A-Reverse | GGATTAGGGCTTCCTCTTGGA |
| RHOH Forward | GAGAAGTAACATTCTGCAAATCGC |
| RHOH Reverse | AGCACACGCCATTCAGCAAG |
| XIST-Forward | AGGTCAGGCAGAGGAAGTCA |
| XIST-Reverse | AGGTCAGGCAGAGGAAGTCA |
| ROX1-AS1 Forward | CTAGTTAGCAGGGGCAGCAC |
| PROX1-AS1 Reverse | AACAGAGAGGCGTGGAAGAA |
| MYC-Forward | TGGACATCCGCAAAGACCTGTAC |
| MYC-Reverse | TCAGGAGGAGCAATGATCTTGA |
| SFTA1P-Forward | CAGCATTCCAGGTGGGCTTT |
| SFTA1P-Reverse | CCTTGTTTGGCTTACTCGTGC |
qPCR data of relative gene expression was analyzed using the ΔΔCt method whereby the endogenous control Ct is subtracted from the gene of interest Ct to calculate ΔCt, and a relative quantity value was calculated by finding 2−ΔΔCt. A Student’s unpaired t-test was used to calculate statistical significance (p < 0.05).