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. 2020 Dec 1;10(12):1621. doi: 10.3390/biom10121621

Figure 1.

Figure 1

TGF-β1-induced EMT in NMuMG mammary epithelial cells is associated with repression of Glut1 and reduced glucose uptake. (A,B) Western blot analysis of the effects of short-term TGF-β1 exposure (10 ng/mL, 72 h) on the expression of the EMT markers CXADR, E-cadherin and vimentin (A), and Glut1 (B) in NMuMG cells. Calnexin was used as a loading control. (C) Immunofluorescence staining of Glut1 (red) in NMuMG cells that were non-treated or treated with TGF-β1 (10 ng/mL, 72 h). DAPI staining was used to visualize cell nuclei. Scale bar = 10 μm. (D) Bar graph showing the effects of TGF-β1 (10 ng/mL, 72 h) on uptake of 2-NBDG in NMuMG cells. *** p < 0.001.