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. 2020 Dec 1;10(12):1621. doi: 10.3390/biom10121621

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glut1 regulates cell proliferation and TGF-β1-induced EMT in mammary epithelial cells. (A) Bar graph showing the effect of transection of scrambled control (Scr) or siRNA against Glut1 (siGlut1) on the proliferative capacity of NMuMG cells. (B) Western blot analysis showing the effect of scrambled control (Scr) or Glut1 siRNA (siGlut1) on TGF-β1-induced changes in the expression of E-cadherin and vimentin during the induction of EMT in NMuMG cells. (C) Line graph showing the effect of lentivirus-mediated overexpression of Glut (RFP-Glut1) or control (RFP) on the viability of NMuMG cells after exposure to different doses of TGF-β1 for 72 h. (D) Western blot analysis showing the effect of overexpression of Glut (RFP-Glut1) or control (RFP) on TGF-β1-induced changes in the expression of E-cadherin and vimentin during the induction of EMT in NMuMG cells. * p < 0.05.