Table 5.
Liposome characteristics of OVA-peptide-containing liposomes. Liposomal size, PDI and zeta potential were measured in 10 or 1 mM HEPES saline buffer, pH 7.4, at 25 °C, respectively. Peptide loading efficiency and capacity were analyzed using fluorescent 5-FAM-Lys-OVA247–279.
| GM3 Amount Incorporated (mol%) | Mean Size (nm) | Mean PDI | Mean Zeta-Potential (mV) | Loading Efficiency (%) | Loading Capacity (ng Peptide/nmol Phospholipid) |
|---|---|---|---|---|---|
| 0 mol% GM3 | 231 ± 6.1 | 0.2 ± 0.05 | −64.8 ± 2.3 | 0.40 ± 0.01 | 2.40 ± 0.04 |
| 3 mol% GM3 | 224 ± 15.5 | 0.2 ± 0.05 | −44.5 ± 1.8 | 0.43 ± 0.02 | 2.41 ± 0.09 |