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. 2020 Dec 1;9(12):2573. doi: 10.3390/cells9122573

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of TMZ-resistant gene candidates and pathways with the knockout library. (A) Plot of enriched CRISPR Knock-Out (GeCKO) library individual sgRNA in the TMZ-treated samples compared to vehicle (DMSO) samples determined by MAGeCK-VISPR tool analysis. (B) Cellular pathway enrichment of TMZ treated samples compared to DMSO treated samples as determined by DAVID tool analysis.