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Validation of knockout library gene candidates. (A–C) RT-PCR of MSH2, CLCA2, and PTCH2 genes in U138MG cells transfected with esiEGFP or esi of gene of interested. (D–F) Dose-response curves of U138MG cells submitted to gene silencing (MSH2, CLCA2, PTCH2, or EGFP), treated with increasing concentrations of TMZ (50 to 500 µM) and analyzed after 120 h treatment using the XTT assay. * p < 0.05, ** p < 0.01, **** p < 0.0001.
