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. 2020 Nov 24;9(12):1173. doi: 10.3390/antiox9121173

Figure 2.

Figure 2

Effects of HAW on the CCl4-induced liver fibrosis rat model. (A) representative images of Masson staining (blue area) staining, 100× magnification, scale bar equals 200 µm. (B) Representative images of immunohistochemically expression of collagen-1 (brown area). Scale bar equals 100 µm, (C) severity score of Masson, and (D) Masson-positive area expression as a percentage of the total area (n = 5 per group) using the free software NIH ImageJ. (E) collagen-1 expression area as a percentage of the total area (n = 6 per group). (F) hydroxyproline (HP) level in the liver. (G) representative images of immunohistochemically expression of collagen-3 in the liver (brown area) scale bar equals 100 µm. (H) collagen-3 expression area as a percentage of the total area (n = 3 per group). (I,J) Analysis of type 1 and 3 collagen mRNA expression in the liver by quantitative RT-PCR (n = 3 per group). Results were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. Data are presented as means ± SEM (n = 6 in each group). a is p < 0.05 vs. control group, b is p < 0.05 vs. CCl4 group, and c is p < 0.05 vs. HAW group.