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. 2020 Dec 1;12(12):3597. doi: 10.3390/cancers12123597

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Abemaciclib treatment regulates expression of cell cycle and DNA repair genes. (A) SNU-EP2 and SNU-EP1203 cells were treated with abemaciclib (0.25 µM) for the indicated times and the levels of phosphorylated RB, AKT, and ERK were detected via Western blot analysis. (B) SNU-EP2 cells were treated with abemaciclib for 48 h and mRNA-seq was performed. A volcano plot showed significantly upregulated (red) and downregulated (blue) genes. (C) Significantly downregulated pathways analyzed by GSEA are listed. (D,E) Expression of cell cycle and DNA repair genes in SNU-EP2 and SNU-EP1203 cells was validated by qPCR (D) and Western blot analysis (E). qPCR results are presented as the mean ± SD for duplicate samples. ** p < 0.01.