Table 2.
Concentrations of flavonoids, secoiridoids, and total phenols (mg/100 g DW) in leaves of six olive cultivars collected at three different periods (CP1—October 2017, CP2—January 2018, and CP3—March 2018) in two locations.
| Factor | Flavonoids (mg/100 g DW) |
Secoiridoids (mg/100 g DW) | Total Phenols (mg/100 g DW) | |||||
|---|---|---|---|---|---|---|---|---|
| Apigenin | Apigenin-7-O- glucoside |
Luteolin | Luteolin-7-O- glucoside |
Rutin | Catechin | Oleuropein | ||
| Cultivar (Cv.) | ||||||||
| Drobnica | 4.92 ± 0.79 b | 29.87 ± 2.96 c | 30.48 ± 3.04 b | 296.78 ± 30.19 d | 55.70 ± 5.77 b | 25.87 ± 3.99 b | 3214.87 ± 722.32 b | 4431.7 ± 224.82 a |
| Istarska bjelica | 2.73 ± 0.71 b | 34.05 ± 1.89 c | 20.59 ± 2.79 c | 323.39 ± 23.55 cd | 107.60 ± 11.67 a | 27.15 ± 4.40 b | 4497.35 ± 705.29 a | 4385.06 ± 213.58 a |
| Lastovka | 4.64 ± 1.12 b | 31.14 ± 2.38 c | 28.49 ± 3.40 bc | 326.72 ± 23.98 cd | 19.82 ± 2.98 d | 25.51 ± 2.97 b | 2307.22 ± 515.42 b | 3714.59 ± 178.10 b |
| Leccino | 10.33 ± 2.25 a | 91.75 ± 5.67 a | 36.49 ± 3.03 b | 382.37 ± 17.13 bc | 55.88 ± 4.48 b | 22.76 ± 2.49 b | 3258.03 ± 476.23 b | 4030.79 ± 217.40 ab |
| Levantinka | 10.28 ± 1.91 a | 62.85 ± 5.09 b | 53.74 ± 3.00 a | 485.70 ± 44.67 a | 35.66 ± 3.96 c | 24.68 ± 3.83 b | 2612.49 ± 549.09 b | 4414.42 ± 165.97 a |
| Oblica | 2.74 ± 0.60 b | 27.79 ± 1.26 c | 31.68 ± 2.36 b | 428.80 ± 25.52 ab | 28.29 ± 3.31 cd | 34.39 ± 5.69 a | 2472.81 ± 493.50 b | 3836.30 ± 191.08 b |
| p-value | *** | *** | *** | *** | *** | *** | *** | *** |
| F-value | 22.09 | 155.49 | 30.88 | 23.12 | 77.75 | 8.75 | 11.58 | 8.6 |
| Collecting period (CP) | ||||||||
| CP1 | 10.60 ± 1.43 a | 41.83 ± 3.88 b | 44.26 ± 2.37 a | 301.23 ± 17.44 b | 43.16 ± 5.95 b | 10.72 ± 1.08 c | 871.71 ± 149.51 c | 3721.92 ± 147.90 b |
| CP2 | 5.34 ± 0.66 b | 43.28 ± 4.70 b | 33.01 ± 2.29 b | 406.60 ± 24.38 a | 55.69 ± 7.17 a | 28.60 ± 1.78 b | 3079.25 ± 376.50 b | 3909.95 ± 118.95 b |
| CP3 | 1.89 ± 0.29 c | 53.63 ± 5.20 a | 23.47 ± 2.13 c | 414.06 ± 21.44 a | 52.63 ± 6.04 a | 40.86 ± 2.71 a | 5230.42 ± 332.04 a | 4774.57 ± 106.21 a |
| p-value | *** | *** | *** | *** | ** | *** | *** | *** |
| F-value | 69.27 | 19.32 | 53.68 | 35 | 6.68 | 248.14 | 169.68 | 53.62 |
| Location (L) | ||||||||
| Pag | 4.49 ± 0.59 b | 52.90 ± 4.24 a | 32.81 ± 2.29 | 455.41 ± 17.20 a | 63.49 ± 6.22 a | 33.38 ± 2.38 a | 3950.63 ± 398.44 a | 4158.72 ± 142.94 |
| Zadar | 7.39 ± 1.09 a | 39.58 ± 3.10 b | 34.34 ± 2.05 | 292.51 ± 12.33 b | 37.49 ± 3.19 b | 20.08 ± 1.89 b | 2170.29 ± 227.20 b | 4112.24 ± 91.12 |
| p-value | *** | *** | n.s. | *** | *** | *** | *** | n.s. |
| F-value | 22.65 | 62.08 | 0.87 | 174.94 | 79.27 | 143.2 | 84.92 | 0.28 |
| Cv. × CP | *** | *** | n.s. | * | n.s. | *** | *** | * |
| F-value | 4.96 | 7.87 | 1.22 | 2.04 | 1.25 | 11.50 | 4.21 | 2.19 |
| Cv. × L | n.s. | *** | n.s. | *** | *** | ** | * | * |
| F-value | 1.48 | 6.09 | 1.36 | 5.48 | 21.81 | 3.46 | 3.08 | 2.76 |
| CP × L | *** | *** | * | n.s. | n.s. | ** | *** | *** |
| F-value | 16.14 | 7.99 | 4.50 | 0.12 | 1.57 | 7.23 | 16.98 | 52.73 |
| Cv. × CP × L | ** | n.s. | n.s. | n.s. | n.s. | *** | *** | * |
| F-value | 2.8 | 1.02 | 1.22 | 0.72 | 0.98 | 3.55 | 3.68 | 2.53 |
Results are expressed as means ± standard errors. Different superscript lowercase letters in a column represent statistically significant differences between mean values for each main effect at p < 0.05 obtained by a two-way ANOVA and Tukey’s test. First (Cv. × CP, Cv. × L, CP × L) and second order interactions (Cv. × CP × L) are presented. Significance: ***—p < 0.001, **—p < 0.01, *—p < 0.05. DW—dry weight.