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. 2020 Nov 27;9(12):428. doi: 10.3390/biology9120428

Figure 1.

Figure 1

Chromodomain-helicase-DNA-binding (CHD)9 is dispensable for the pluripotency of embryonic stem cells (ESCs). (a) Expression values of the CHD family (Chd1 to Chd9) in the ESCs and differentiated cells after 2, 4, and 6 d using RNA-Seq data (GSE114219). FPKM; Fragment per kilobase of transcript per million mapped reads. (b) shRNA-mediated Chd9 KD efficiency in the ESCs was confirmed using qRT-PCR (n = 3). (c) ESCs were stained to screen for pluripotency through alkaline phosphatase activity. (d,e) The expression of the pluripotent markers (NANOG, OCT3/4, SOX2, and SSEA-1(IF)) was determined via immunofluorescence staining (d) and qRT-PCR (e), respectively. There was no significant difference in the transcription of Nanog, Oct3/4, and Sox2 upon Chd9 KD. The ESCs were immunostained with anti-NANOG, OCT3/4, SOX2, and SSEA-1 antibodies; scale bars in the merged images of low magnification in the left panels are 200 μm and of high magnification in the right panels are 100 μm.