Table 1.
Potential methods to prepare liposomes.
| Preparation Methods | Subtypes | Comments |
|---|---|---|
| Mechanical dispersion methods | probe or bath sonication | − the critical parameters vary on the basis of the selected preparation method; therefore, the definition of the production technique has to be the first step of every liposome formulation process − the properties of the liposomes (e.g., number of lamellas, size, and distribution of vesicles) |
| French pressure cells—extrusion | ||
| freeze-thawed liposomes | ||
| membrane extrusion | ||
| lipid film hydration techniques | ||
| hydration of proliposomes | ||
| micro emulsification, coalescence of small vesicles | ||
| dual asymmetric centrifugation | ||
| heating method, Mozafari method | ||
| electro-formation | ||
| Solvent dispersion methods | ether injection | |
| ethanol injection | ||
| reverse-phase evaporation | ||
| solvent spherule method | ||
| Detergent removal methods | dialysis | |
| detergent removal of mixed micelles | ||
| gel-permeation chromatography | ||
| Novel methods | microfluidisation | |
| supercritical-assisted method | ||
| freeze-drying of double emulsions | ||
| membrane contractor method | ||
| curvature-tuning | ||
| biometric reaction for vesicular self-assembly |