Figure 4.

Culture parameter optimization of YL-1 polysaccharide production. (a) culture temperature; (b) rotator speed; (c) culture time. Culture temperature was adjusted as 22, 24, 26, 28, 30 °C. Rotator speed was adjusted as 100, 120, 140, 160, 180, 200 rpm. Culture time optimization was made by collecting samples at intervals of 12 h. All tests were performed in triplicate and repeated at least once, and the results were expressed by their means ±SD (standard deviation). Statistical significance of the treatment effects was determined by Duncan’s multiple range t-test.