Table 2.
Methylation analysis of polysaccharide. (Data were analyzed according to the GC–MS chromatogram (peaks) shown in Figure 7.
| RT | Methylated Sugar | Mass Fragments (m/z) | Molar Ratio | Type of Linkage |
|---|---|---|---|---|
| 12 | 2,3,4-Me3-Fucp | 43, 59, 72, 89, 101, 115, 117, 131, 175 | 1.17 | Fuc-(1→ |
| 16.26 | 2,3,4,6-Me4-Glcp | 43, 71, 87, 101, 117, 129, 145, 161, 205 | 15 | Glc-(1→ |
| 16.556 | 2,3,4,6-Me4-Manp | 43, 71, 87, 101, 117, 129, 145, 161, 205 | 5.51 | Man-(1→ |
| 22.216 | 2,4,6-Me3-Galp | 43, 87, 99, 101, 117, 129, 161, 173, 233 | 52.8 | →3)-Gal-(1→ |
| 22.608 | 2,3,4-Me3-Manp | 43, 71, 87, 99, 101, 117, 129, 159, 161 | 3.84 | →6)-Man-(1→ |
| 25.074 | 4,6-Me2-Manp | 43, 85, 87, 99, 101, 127, 129, 161, 201, 216 | 5.36 | →2,3)-Man-(1→ |
| 29.548 | 2,4-Me2-Manp | 43, 87, 117, 129, 159, 189, 233 | 12.38 | →3,6)-Man-(1→ |
| 27.311 | 2,3-Me2-Glcp | 43, 71, 85, 87, 99, 101, 117, 127, 159, 161, 201 | 3.94 | →4,6)-Glc-(1→ |