Table 1.

Relative hepatic CYP2E1-, Insig 1/2- and SREBP1c-content and MDA-levels of CHIP- and gp78-knockout (KO) mice.

Parameter	CHIP		gp78
	WT	KO	WT	KO
CYP2E1	100	213.0	100	155.7 b
MDA	100 ± 3.4 a	169.9 ± 13.9 a	100 ± 2.2	109.9 ± 4.7
Insig-1	100	36.2	100	329.4
Insig-2	100	41.5	100	346.1
SREBP1 (P)	100	208.4	100	82.2
SREBP1 (N)	100	151.4	100	72.9

Hepatocytes were isolated from livers of wild type (WT_ and corresponding CHIP^−/− mice or WT and corresponding gp78^−/− mice, aged 8–9 weeks, and cultured for 48 h before treatment with the CYP2E1 inducer INH for the next 72 h. Lysates were prepared and aliquots subjected to Western immunoblotting analyses with GAPDH as the loading control. Malondialdehyde (MDA) levels were monitored as described as markers of hepatic lipid peroxidation. Values (Mean of 2 individual mouse livers) were normalized to the GAPDH content and expressed as % of the corresponding WT-content. P, N, precursor and nuclear species, respectively, identified on the basis of their relative molecular masses. Original immunoblotting data were reported [34]. ^a MDA-levels in similarly cultured WT and CHIP^−/−-mouse hepatocytes are included for reference and were derived from our previous report [29]. ^b Although the mean CYP2E1-content in the gp78^−/−-hepatocytes used to concurrently monitor Insig1/2- and SREBP-content was lower than in CHIP^−/−-hepatocytes, this pertained largely to these two sets of 2 animals each examined concurrently. However, the bulk of our data in our other studies [29,33] revealed the hepatic CYP2E1 content to be quite comparable in both KO-models, ranging between 155.7 and 221.3% of corresponding WT-controls in gp78^−/−-hepatocytes.