Figure 3.

NGS-MRD markers for relapse detection in relapsed patients with MC fluctuations. NGS-MRD during MC monitoring helps to anticipate clinical relapse. Detection of positive NGS-MRD anticipates relapse 220 days in UPN12 (A) and 40 days in UPN19 (B). Post-HSCT engraftment analysis by indel-qPCR results are plotted as percentage of receptor (Y-axis) over time shown as days post-HSCT (X-axis). Vertical dotted lines denote the NGS-analysis time points and the height bars represents VAF percentages; asterisk indicate NGS-MRD variants. (NGS = next generation sequencing; MRD = minimal residual disease; MC = mixed chimerism; CC = complete chimerism; HSCT = hematopoietic stem cell transplant; UPN = unique patient number; VAF = variant allele frequency).