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MT-III induces production of PGE2, PGI2, TXA2 and LTB4 by 3T3-L1 preadipocytes. Cells were incubated with MT-III (0.4 µM) or DMEM (control) for 1 to 48 h. Bar graphs show the MT-III-induced release of PGE2 (A), PGI2 (B), TXA2 (C) and LTB4 (D) by preadipocytes. Concentrations were quantified in culture supernatants by EIA commercial kit. Results are expressed as mean ± SEM from 3 independent experiments. * p < 0.05 as compared with control group (two-way ANOVA and Bonferroni posttest).
