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MT-III-induced PGE2 release is dependent on cPLA2-α in 3T3-L1 preadipocytes. Cells were incubated with Pyr-2 (1 μM) for 1 h followed by incubation with MT-III (0.4 µM) for 3 h. PGE2 concentrations were quantified in culture supernatants by EIA commercial kit. * p < 0.05 as compared with control group and # p < 0.05 as compared with MT-III group (two-way ANOVA and Bonferroni posttest).
