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. 2020 Nov 24;10(12):1593. doi: 10.3390/biom10121593

Figure 5.

Figure 5

Figure 5

EP4 receptor participates in MT-III-induced PGE2 biosynthesis in 3T3-L1 preadipocytes. (A) Preadipocytes were incubated with SC-19220 (10 μM), AH6809 (10 μM), L-798106 (1 μM) or AH23848 (10 μM) for 1 h followed by incubation with MT-III (0.4 µM) for 6 h. PGE2 concentrations were quantified in culture supernatants by EIA commercial kit. (B–I) 3T3-L1 cells were incubated with MT-III (0.4 µM) or DMEM (control) for 1 up to 48 h. (B,D,F,H) Western blotting of EP1, EP2, EP3 and EP4 receptors, respectively, and β-actin (loading control), showing immunoreactive bands. (C,E,G,I) Densitometric analysis of immunoreactive bands for EP1, EP2, EP3 and EP4 receptors, respectively. Results are expressed as mean ± SEM from 3 independent experiments. * p < 0.05 as compared with control group and # p < 0.05 as compared with MT-III group (one-way ANOVA and Bonferroni posttest in (A) and two-way ANOVA and Bonferroni posttest in (C,E,G,I)).