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MT-III upregulates gene expression of leptin and adiponectin by 3T3-L1 preadipocytes. Cells were incubated with MT-III (0.4 μM) or DMEM (control) for 1, 3 or 6 h. Graphs show gene expression of leptin (A), adiponectin (B) and resistin (C) in the presence of MT-III. Concentrations of adipokines were quantified in cell lysates by qPCR. Results are expressed as mean ± SEM from 5 experiments. * p < 0.05 as compared with the control group (two-way ANOVA and Bonferroni posttest).
