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Schematic representation of 3D cell culture model preparation. (1) Gelatin methacrylate synthesis; (2) valvular interstitial cells (VIC) encapsulation in hydrogel (2 × 106 cells/mL); (3) Cross-linking by ultraviolet (UV) exposure; (4) valvular endothelial cells (VEC) seeding on top of the cross-linked hydrogel.
