Table 3.
CAZome of bacterial isolates for the major glycoside hydrolase (GH) families involved in degradation of plant cell walls.
| CAZy Family | Major Known Activity |
B. theta VPI-5482 |
B. theta CMU13 |
B. theta CMU108 |
B. ovatus ATCC8483 |
B. ovatus CMU19 |
B. ovatus CMU33 |
B. fragilis CMU36 |
B. fragilis CMU103 |
|---|---|---|---|---|---|---|---|---|---|
| Cellulose- and pectin-active | |||||||||
| GH28 | galacturonases | 10 | 10 | 10 | 14 | 15 | 15 | 0 | 0 |
| GH95 | α-L-fucosidase | 5 | 5 | 5 | 7 | 9 | 9 | 4 | 4 |
| GH88 | β-glucouronyl hydrolase | 4 | 3 | 3 | 8 | 6 | 6 | 1 | 1 |
| GH5 | cellulase | 0 | 1 | 1 | 5 | 7 | 7 | 1 | 1 |
| GH53 | endo-1,4-β-galactanase | 1 | 1 | 1 | 1 | 2 | 2 | 0 | 0 |
| GH10 | endo-1,4-β-xylanase | 0 | 0 | 0 | 7 | 3 | 3 | 0 | 0 |
| GH9 | endoglucanase | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 |
| GH11 | xylanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH12 | endoglucanase & xyloglucan hydrolysis | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH26 | β-mannanase & xylanase | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 2 |
| GH44 | endoglucanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH45 | endoglucanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH48 | endo-processive cellulases | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH6 | endoglucanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH7 | endoglucanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH8 | endo-xylanases | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 20 | 20 | 20 | 46 | 43 | 43 | 8 | 8 | |
| Cell wall elongation | |||||||||
| GH16 | xyloglucanases & xyloglycosyltransferases | 3 | 4 | 4 | 4 | 7 | 7 | 4 | 4 |
| GH74 | endoglucanases & xyloglucanases | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
| GH17 | 1,3-β-glucosidases | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH81 | 1,3-β-glucanase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 3 | 5 | 5 | 4 | 7 | 7 | 5 | 5 | |
| Debranching enzymes | |||||||||
| GH78 | α-L-rhamnosidase | 6 | 9 | 9 | 8 | 9 | 9 | 2 | 2 |
| GH106 | α-L-rhamnosidase | 3 | 4 | 4 | 4 | 4 | 4 | 0 | 0 |
| GH51 | α-L-arabinofuranosidase | 4 | 4 | 4 | 4 | 4 | 4 | 2 | 2 |
| GH23 | peptidoglycan lyase | 3 | 4 | 3 | 3 | 3 | 3 | 3 | 3 |
| GH33 | trans-sialidase | 2 | 2 | 2 | 5 | 5 | 5 | 4 | 4 |
| GH146 | β-L-arabinofuranosidase | 3 | 2 | 2 | 3 | 3 | 3 | 0 | 0 |
| GH27 | α-galactosidase | 5 | 2 | 2 | 3 | 2 | 2 | 2 | 2 |
| GH77 | 4-α-glucanotransferase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| GH84 | N-acetyl β-glucosaminidase | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 |
| GH67 | α-glucuronidase | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 0 |
| GH103 | peptidoglycan lytic transglycosylase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH54 | α-L-arabinofuranosidase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH62 | α-L-arabinofuranosidase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 29 | 29 | 28 | 33 | 33 | 33 | 15 | 15 | |
| Oligosaccharide-degrading enzymes | |||||||||
| GH2 | β-galactosidases and other β-linked dimers | 32 | 34 | 34 | 37 | 32 | 32 | 15 | 15 |
| GH43 | arabinases & xylosidases | 34 | 33 | 33 | 35 | 39 | 39 | 10 | 10 |
| GH92 | α-1,2-mannosidase | 23 | 26 | 26 | 18 | 20 | 20 | 8 | 8 |
| GH20 | β-hexosaminidase | 14 | 13 | 13 | 13 | 11 | 11 | 11 | 11 |
| GH3 | mainly β-glucosidases | 10 | 11 | 11 | 21 | 23 | 23 | 10 | 10 |
| GH105 | unsaturated rhamnogalacturonyl hydrolase | 7 | 9 | 9 | 12 | 15 | 15 | 0 | 0 |
| GH18 | chitinase | 12 | 9 | 9 | 8 | 10 | 10 | 2 | 2 |
| GH97 | glucoamylase, α-glucosidase, α-galactosidase | 10 | 9 | 9 | 12 | 9 | 9 | 4 | 4 |
| GH29 | α-L-fucosidase | 9 | 9 | 9 | 7 | 5 | 5 | 9 | 9 |
| GH130 | β-1,4-mannosylglucose phosphorylase | 4 | 6 | 6 | 8 | 6 | 6 | 2 | 2 |
| GH31 | α-glucosidase | 6 | 6 | 6 | 12 | 6 | 6 | 4 | 4 |
| GH35 | β-galactosidase | 3 | 4 | 4 | 2 | 5 | 5 | 3 | 3 |
| GH32 | invertase, endo-inulinase | 4 | 4 | 4 | 2 | 3 | 3 | 2 | 2 |
| GH38 | α-mannosidase | 2 | 4 | 4 | 1 | 1 | 1 | 1 | 1 |
| GH127 | β-L-arabinofuranosidase | 3 | 3 | 3 | 1 | 4 | 4 | 5 | 5 |
| GH13 | α-amylase | 8 | 2 | 2 | 5 | 2 | 2 | 2 | 2 |
| GH141 | α-L-fucosidase, xylanase | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 1 |
| GH27 | α-galactosidase | 5 | 2 | 2 | 3 | 2 | 2 | 2 | 2 |
| GH137 | β-L-arabinofuranosidase | 1 | 2 | 2 | 1 | 1 | 1 | 1 | 1 |
| GH42 | β-galactosidase | 1 | 1 | 1 | 1 | 3 | 3 | 0 | 0 |
| GH138 | α-galacturonidase | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| GH139 | α-2-O-Me-L-fucosidase | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| GH142 | β-L-arabinofuranosidase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| GH143 | 2-keto-3-deoxy-D-lyxo-heptulosaric acid hydrolase | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| GH57 | α-amylase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| GH147 | β-galactosidase | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 |
| GH1 | β-glucosidase and many other β-linked dimers | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH39 | β-xylosidase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH52 | β-xylosidase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| GH94 | cellobiose phosphorylase | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 195 | 194 | 194 | 207 | 206 | 206 | 94 | 94 | |
Underlined and bolded values highlight CAZy families with more members in the CM isolates than the wild-type strains (B. theta VPI-5482 and B. ovatus ATCC8483).