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. 2020 Nov 30;12(12):3575. doi: 10.3390/cancers12123575

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Plasma treatment inactivated skin cancer cells in a treatment time-dependent fashion. (a) atmospheric pressure argon plasma jet kINPen; (b) representative brightfield image of the two cancer cell types used in this study; (c,d) normalized metabolic activity 4 h and 24 h after plasma treatment in A431 (c) and A375 (d) cells; (e) representative overlay flow cytometry histograms of the terminal cell death dye DAPI in cells with and without plasma treatment at 24 h; (f–h) quantification of viable A431 (f), A375 (g), and HaCaT (h) cells using flow cytometry. Data are the mean of three independent experiments. Statistical analysis was performed using one-way ANOVA (* = p < 0.01, ** = p < 0.01, *** = p < 0.001). ns = not significant, ctr = control.