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. 2020 Nov 30;12(12):3575. doi: 10.3390/cancers12123575

Figure 2.

Figure 2

Plasma treatment modulated NK-cell ligand-receptor expression predominantly in malignant cells. (a–d) representative overlay flow cytometry histograms of MIC A,B (a) and HLA-A,B,C (b), and quantification and normalization of the MFI of MIC A,B (c) and HLA-A,B,C (d) in viable A431 and A375 cells at 4 h and 24 h after plasma treatment; (e–h) representative overlay flow cytometry histograms of HLA-E (e) and PD-L1 (f), and quantification and normalization of the MFI of HLA-E (g) and PD-L1 (h) in viable A431 and A375 cells at 4 h and 24 h after plasma treatment; (i,j) representative overlay flow cytometry histograms of MIC A,B, PD-L1, HLA-E, and HLA-A,B,C (i) and quantification and normalization of their corresponding MFI (j) in viable HaCaT cells at 4 h and 24 h after plasma treatment. Data are the mean of three independent experiments. Statistical analysis was performed using one-way ANOVA (* = p < 0.01, ** = p < 0.01, *** = p < 0.001). MFI = mean fluorescent intensity.