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In vitro reconstitution of the FtsZ/FtsA interaction. FtsZ was induced to polymerize in the presence or absence of FtsA and GTP or GDP. The co-sedimentation was carried out in the presence of vesicles. The polymeric FtsZ and FtsA were collected by sedimentation and the amount of proteins in the pellet (P) was estimated by coomassie-blue staining of SDS-PAGE. S, supernatant.
