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. 2020 Nov 26;9(12):421. doi: 10.3390/biology9120421

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Auxin-inducible degradation (AID). The AID system requires the ectopic expression of both the OsTIR1 gene and aid-tagged protein of interest (POI). As shown in the figure, the exogenous OsTIR1 gene can form a functional transgenic SCF complex by connecting with the endogenous elements of the complex (Skp1, Cul1 and Rbx1). When auxin is added in the culture medium, it binds OsTIR1 triggering POI recognition and its subsequent polyubiquitination by an E2 ubiquitin-conjugating enzyme. The tagged protein is rapidly lead to the proteasome for degradation. POI: protein of interest. Ub: ubiquitin.