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. 2020 Nov 27;13(12):429. doi: 10.3390/ph13120429

Figure 2.

Figure 2

ERRγ is an inducer of MMP-3 and MMP-13 in articular chondrocytes. Primary cultured articular chondrocytes were exposed to IL-1β (0–1 ng/mL) (A), IL-6 (0–50 ng/mL) (B), and TNF-α (0–50 ng/mL) (C) for 24 h. ERRγ protein levels were quantified by Western blotting. (D) mRNA levels of ERRγ and catabolic factors (MMP-3, MMP-12, MMP-13, and ADAMTS5) were analyzed by RT-PCR and qRT-PCR in primary cultured chondrocytes infected with Ad-C (800 MOI) or the indicated MOI of Ad-Esrrg for 36 h. (E) The protein levels of ERRγ, MMP-3, and MMP-13 were analyzed by Western blot with semi-quantification in primary cultured chondrocytes infected with Ad-C (800 MOI) or the indicated MOI of Ad-Esrrg for 36 h. (F) The protein levels of ERRγ, MMP-3, and MMP-13 were analyzed by Western blotting with semi-quantification in primary cultured chondrocytes infected with Ad-Esrrg (800 MOI) for the indicated hours. The results are representative of three independent experiments from different pups. Values are presented as mean ± SEM (* p < 0.05, ** p < 0.01, and *** p < 0.001). One-way ANOVA. GAPDH, β-actin, and Lamin B were used as internal markers. ERRγ, estrogen-related receptor γ; IL-1β, interleukin-1β, IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; MOI, multiplicity of infection; MMP-3, matrix metalloproteinase-3; MMP-13, matrix metalloproteinase-13.