Table 1.
Genes | Strand | Primer Sequences | Size (bp) |
AT (°C) |
Origin |
---|---|---|---|---|---|
ADAMTS5 | S AS |
5′-GCCATTGTAATAACCCTGCACC-3′ 5′-TCAGTCCCATCCGTAACCTTTG-3′ |
292 | 58 | Mouse |
Esrrg | S AS |
5′-AAGATCGACACATTGATTCCAGC-3′ 5′-GCTTCACATGATGCAACCCC-3′ |
350 | 64 | Mouse |
GAPDH | S AS |
5′-TCACTGCCACCCAGAAGAC-3′ 5′-TGTAGGCCATGAGGTCCAC-3′ |
450 | 58 | Mouse |
MMP-3 | S AS |
5′-AGGGATGATGATGCTGGTATGG-3′ 5′-CCATGTTCTCCAACTGCAAAGG-3′ |
434 | 58 | Mouse |
MMP-12 | S AS |
5′-CCCAGAGGTCAAGATGGATG-3′ 5′-GGCTCCATAGAGGGACTGAA-3′ |
482 | 60 | Mouse |
MMP-13 | S AS |
5′-TGATGGACCTTCTGGTCTTCTGG-3′ 5′-CATCCACATGGTTGGGAAGTTCT-3′ |
473 | 58 | Mouse |
AT, annealing temperature; S, sense; AS, antisense.