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. 2020 Nov 27;13(12):429. doi: 10.3390/ph13120429

Table 1.

PCR primers and conditions.

Genes Strand Primer Sequences Size
(bp)
AT
(°C)
Origin
ADAMTS5 S
AS
5′-GCCATTGTAATAACCCTGCACC-3′
5′-TCAGTCCCATCCGTAACCTTTG-3′
292 58 Mouse
Esrrg S
AS
5′-AAGATCGACACATTGATTCCAGC-3′
5′-GCTTCACATGATGCAACCCC-3′
350 64 Mouse
GAPDH S
AS
5′-TCACTGCCACCCAGAAGAC-3′
5′-TGTAGGCCATGAGGTCCAC-3′
450 58 Mouse
MMP-3 S
AS
5′-AGGGATGATGATGCTGGTATGG-3′
5′-CCATGTTCTCCAACTGCAAAGG-3′
434 58 Mouse
MMP-12 S
AS
5′-CCCAGAGGTCAAGATGGATG-3′
5′-GGCTCCATAGAGGGACTGAA-3′
482 60 Mouse
MMP-13 S
AS
5′-TGATGGACCTTCTGGTCTTCTGG-3′
5′-CATCCACATGGTTGGGAAGTTCT-3′
473 58 Mouse

AT, annealing temperature; S, sense; AS, antisense.