Figure 4.

Relative sphingolipid levels in cells expressing respective human GBA2 isoforms. (A) The heat map illustrates Z-score differences from mean of sphingolipid in cells expressing the human GBA2 isoforms and control at 48 h after transfection, while the cluster maps illustrate the similarities of the patterns. The z-scores are color-coded from blue (lower than average for that lipid species) to red (higher than average for that lipid species), (B) ceramide (34:1, 36:1, 40:1, 40:2, 42:1, 42:2, and 42:3), (C) hexosylceramide (34:1, 40:1, 42:1, and 42:2), and (D) sphingomyelin (36:2, 40:1, 40:2, 41:1, 41:2, 42:1, 42:2, 42:3, 44:2, and 44:3) expressed as bar graphs. Values are means of three independent biological replicates with standard deviations shown as error bars, * p < 0.05 and ** p < 0.01 for differences compared to empty vector control in a two-tailed unpaired t-test. Shapiro-Wilk analysis showed no significant deviation from a normal distribution for those conditions showing significant differences.